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RESEARCH ARTICLE                      BIOSCIENCE RESEARCH, 2025 22(2): 119-129.                     OPEN ACCESS

Comparative study on the Therapeutic effects of probiotics mixture on oxidative stress Biomarkers in healthy and fungal-infected Rohu (Labeo rohita)

Rabia Bashir^1*, Razia Kausar^2*, Hasooba Hira¹, Tayyba Batool¹, Mudassar Yasmeen³, Syeda Eishah Tu Razia¹, Zahida Nasreen⁴ and Zarqa Batool⁴.

¹Department of Zoology, Wildlife and Fisheries, University of Agriculture, Faisalabad-38040, Pakistan

²Department of Anatomy, University of Agriculture, Faisalabad-38040, Pakistan

³Department of Biochemistry, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan

⁴Department of Zoology, University of Mianwali, Pakistan                                               

DOI:

Abstract

Aquaculture production is the major source of food requirements to combat with ever growing food security issues and for this reason improving aquaculture production quantitatively along with quality is essential. Probiotics play a vital role in this scenario in different countries of the world. This study aimed to elucidate the effects of blended probiotics on healthy and diseased fish. This experimental trial was conducted in Fisheries Research Center Chashma, Mianwali for 74 days. Total 180 samples were used and grouped according to their average body size. Two different concentrations of probiotics as 2% and 4% were given to all treatment groups. Antioxidant enzymes activity was analyzed in both normal and fungal infected groups. Catalase activity increased significantly from T0 to T2 in both normal (53.7±2.98 to 75.9±2.56) and infected fish (81.1±1.66 to 92.4±5.95). Malondialdehyde (MDA) level showed a progressive rise, with values ranging from 518.7±4.57 to 534±8.12 in normal and 681±9.64 to 704±8.60 in infected fish. Peroxidase activity was evaluated from 698±5.33 (T0) to 728±18.3 (T2) in normal fish and from 1521±7.20 to 1604±31.6 in diseased fish. Superoxide dismutase (SOD) level was also improved ranging from 73.2±1.75 to 83.4±1.65 in normal and 91.3±1.16 to 99.6±2.12 in infected fish. These findings suggested that probiotics supplementation enhances antioxidant defense mechanisms particularly in fungus-infected fish. These results suggested that commercial blended probiotics work more efficiently and effects positively on antioxidant enzymes activity as it offers the promising applications of preventive as well as therapeutic measures in aquaculture.