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RESEARCH ARTICLE
BIOSCIENCE RESEARCH,
2025 22(2): 68-75. OPEN
ACCESS
In-Vitro
Screening of pathogenic bacteria in brown mussels Perna perna of
Al-Mukalla coast, Gulf of Aden, Yemen
Maged S.
Bin-Masalam 1, Maher A. Ba-Amer 1,
Feras S. Bajaba 2,3*and Abdulgader H. Albar 2,4
1
Department
of Food Science and Technology, Faculty of Environmental Sciences and Marine
Biology, Hadhramout University, Al-Mukalla,
Yemen
2
Biological
Sciences Department, Faculty of Science, Microbiology, King Abdulaziz
University, Jeddah, Saudi
Arabia
3
Biology
Department, Collage of Science, University of Jeddah, Jeddah,
Saudi Arabia
4
Department
of Microbiology and Parasitology, Collage of Medicine, University of Jeddah,
Jeddah, Saudi Arabia
DOI:
Abstract
The brown mussel Perna perna was harvested from
two marine stations on the Al-Mukalla coast (Mouth of Khor Al-Mukalla and
Broom) to detect the main load of seasonally pathogenic bacteria. 250
samples of P. perna were collected monthly from October 2018 until
October 2019 and evaluated seasonally. A protocol was designed for the
isolation, enumeration, and partial identification of coliforms,
Escherichia coli, Staphylococcus aureus, Vibrio, and Salmonella typhi.
The results were processed and analyzed; the means with standard deviations
were calculated and then compared. Results showed that the number of
isolated bacteria was generally higher in summer and lower in winter. Based
on MPN, the numbers (CFU g-1of coliforms, E. coli and Staph.
aureus in summer were in the range of 117.0, 131.33 and 11.13 MPN,
respectively, whereas the count recorded in winter were 26.30, 46.30 and
90.37 CFU g-1), respectively. While V. parahaemolyticus and S.
typhi were neglected. For Broom station, the numbers of coliform and
E. coli were in the range of 5.60 - 9.19 and 3.15 - 4.52 MPN g⁻¹,
in winter and summer, respectively. While Staph. aureus, V.
parahaemolyticus, and S. typhi were absent.
Significant statistical differences were found in the
numbers of tested bacteria between two stations
were significant at P<0.05, particularly in
coliform numbers and E. coli numbers from
P. perna
mussels collected from Khor Al-Mukalla and Broom
stations. No significant differences were found in E. coli and
coliform counts, remaining within the Gulf Cooperation Council GSO
acceptable range.
Keywords:
coliform, marine, mussel,
Perna,
Staphylococcus.
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